A Laboratory Manual of Proteome Analysis

by Reiner Westermeier and Tom Naven

Proteomics in Practice combines the best features of a laboratory manual and general reference for the successful analysis of proteins. Among the methods covered are the classical approach of 2-D gel electrophoresis, mass spectrometry and related sequence database inquiries.

Hardback
Pages: xxiv + 318
Published: 2002

Contents

Foreword
Preface
Abbreviations, Symbols, Units
Glossary of Terms

PART I: PROTEOMICS TECHNOLOGY
Introduction
Expression Proteomics
Two-dimensional Electrophoresis
Spot Handling
Mass Spectrometry
Protein Identification by Database Searching
Methods of Proteomics

PART II: COURSE MANUAL
Step 1: Sample Preparation
Step 2: Isoelectric Focusing
Step 3: SDS Polyacrylamide Gel Electrophoresis
Step 4: Staining of the Gels
Step 5: Scanning of Gels and Image Analysis
Step 6: 2D DIGE
Step 7: Spot Excision
Step 8: Sample Destaining
Step 9: In-gel Digestion
Step 10: Microscale Purification
Step 11: Chemical Derivatisation of the Peptide Digest
Step 12: MS Analysis
Step 13: Calibration of the MALDI-ToF MS
Step 14: Preparing for a Database Search
Step 15: PMF Database Search Unsuccessful

Appendices
Trouble Shooting Guide
References