^*Indicates author to whom correspondence should be addressed

Toward efficient analysis of >70 kDa proteins with 100% sequence coverage

Andrew J. Forbes, Matthew T. Mazur and Neil L. Kelleher^*
Department of Chemistry, 600 S. Mathews Avenue, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA E-mail: kelleher@scs.uiuc.edu
Hiten M. Patel and Christopher T. Walsh
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA

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DOI: 10.1255/ejms.393

Gas-phase conformation can have an influence on peptide fragmentation

William J. Griffiths^* and Andreas P. Jonsson
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-17177 Stockholm, Sweden. E-mail: william.griffiths@mbb.ki.se

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DOI: 10.1255/ejms.394

Analysis of protein sequences and protein complexes by matrix-assisted laser desorption/ionization mass spectrometry

Maya Belghazi and Katell Bathany
Institut Européen de Chimie Biologie, 33607 Pessac, France
Codjo Hountondji
Laboratoire de Biochimie, UMR CNRS 7654, Ecole Polytechnique, 91128 Palaiseau, France
Xavier Grandier-Vazeille and Stéphen Manon
Institut de Biologie et Génétique Cellulaire du CNRS, 33077 Bordeaux, France
Jean-Marie Schmitter^*
Laboratoire de Physico-Toxico-Chimie, UMR CNRS 5472 Université Bordeaux I, 33405 Talence, France

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DOI: 10.1255/ejms.395

Simplified sample preparation method for protein identification by matrix-assisted laser desorption/ionization mass spectrometry: in-gel digestion on the probe surface

Allan Stensballe and Ole Nørregaard Jensen^*
Department of Biochemistry & Molecular Biology, University of Southern Denmark, Odense University, Campusvej 55, DK-5230 Odense M, Denmark. E-mail: jenseno@bmb.sdu.dk

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DOI: 10.1255/ejms.396

Improved accuracy in the matrix-assisted laser desorption/ionisation mass spectrometry determination of the molecular mass of cyanogen bromide fragments of proteins by post-cleavage reaction with tris(hydroxymethyl)aminomethane

A. Compagnini, V. Cunsolo, S. Foti and R. Saletti^*
Dipartimento di Scienze Chimiche, Università degli Studi di Catania, Viale A. Doria, 6, 95125 Catania, Italy. E-mail: sfoti@dipchi.unict.it

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DOI: 10.1255/ejms.397

Limited acid hydrolysis as a means of fragmenting proteins isolated upon ProteinChip^® Array surfaces

Shanhua Lin, Pete Tornatore and Scot R. Weinberger^*
Ciphergen Biosystems, Inc., 6611 Dumbarton Circle, Fremont, CA 94555, USA. E-mail: sweinberger@ciphergen.com
Daniel King and Ron Orlando
CCRC, University of Georgia, Athens, GA 30602, USA

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DOI: 10.1255/ejms.398

Integrated system for high-throughput protein identification using a microfabricated device coupled to capillary electrophoresis/nanoelectrospray mass spectrometry

Jianjun Li, Tammy-Lynn Tremblay and Pierre Thibault^*
Institute for Biological Sciences, 100 Sussex Dr., Ottawa, Ontario, Canada K1A 0R6 E-mail: pierre.thibault@nrc.ca
Can Wang, Said Attiya and D. Jed Harrison
Department of Chemistry, University of Alberta, Edmonton, AB, Canada T6G 2G2

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DOI: 10.1255/ejms.399

Investigation of the applicability of a sequential digestion protocol using trypsin and leucine aminopeptidase M for protein identification by matrix- assisted laser desorption/ionization mass spectrometry

Alan Doucette and Liang Li^*
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada, T6G 2G2. E-mail: Liang.Li@ualberta.ca

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DOI: 10.1255/ejms.400

Multiple separations facilitate identification of protein variants by mass spectrometry

Zhongli Zhang, David L. Smith and Jean B. Smith^*
Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA E-mail: jsmith8@unl.edu

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DOI: 10.1255/ejms.401

Mass spectrometric characterization of proteins extracted from Jurkat T cell detergent-resistant membrane domains

Priska D. von Haller, Sam Donohoe, Ruedi Aebersold and Julian D. Watts^*
Department of Molecular Biotechnology, University of Washington, Seattle, WA 98195, USA E- mail: julian@systemsbiology.org
David R. Goodlett
Institute for Systems Biology, 4225 Roosevelt Way, Suite 200, Seattle, WA 98105-6099, USA

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DOI: 10.1255/ejms.402

The effects of transcription regulating genes PDR1, pdr1-3 and PDR3 in pleiotropic drug resistance

Arkadiusz Nawrocki, Stephen J. Fey and Peter Mose Larsen^*
Centre for Proteome Analysis in Life Sciences, International Science Park Odense, University of Southern Denmark, Forskerparken 10B, 5230 Odense M, Denmark. E-mail: ps@cpa.spo.dk
André Goffeau
Chaire Internationale Blaise Pascal, Laboratoire Genetique Moleculaire, Ecole Normale Superieure, 46 rue d’Ulm, 75230 Paris Cedex 05, France
Peter Roepstorff
Department of Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark

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DOI: 10.1255/ejms.403

Structural modifications of Methanococcus jannaschii flagellin proteins revealed by proteome analysis

Carol S. Giometti,^* Sandra L. Tollaksen and Gyorgy Babnigg
Biosciences Division, Argonne National Laboratory, 9700 South Cass Avenue, Building 202, Room B117, Argonne, IL 60439, USA. E-mail: csgiometti@anl.gov
Claudia I. Reich and Gary J. Olsen
Department of Microbiology, University of Illinois at Urbana- Champaign, Urbana, IL, USA
Hanjo Lim and John R. Yates, III
Mass Spectrometry Group, The Scripps Institute, La Jolla, CA, USA

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DOI: 10.1255/ejms.404

Detection and localisation of disulphide bonds in a synthetic peptide reproducing the sequence 1–30 of Par j 1.0101 by electrospray ionisation mass spectrometry

Vincenzo Cunsolo, Salvatore Foti and Rosaria Saletti
Dipartimento di Scienze Chimiche, Università di Catania, Viale A. Doria 6, I-95125 Catania, Italy
Leopoldo Ceraulo^* and Vita di Stefano
Dipartimento di Chimica e Tecnologie Farmaceutiche, Università di Palermo, Via Archirafi 32, I-90123 Palermo, Italy E-mail: lceraulo@unipa.it

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DOI: 10.1255/ejms.405

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