Search for melanoma markers in plasma and serum samples

Eur. J. Mass Spectrom. 11, 353–360 (2005)
DOI: 10.1255/ejms.751

Search for melanoma markers in plasma and serum samples

Roberta Seraglia,^a Susanna Vogliardi,^a Graziella Allegri,^b Stefano Comai,^bMario Lise,^c Carlo Riccardo Rossi,^c Simone Mocellin,^c Romano Scalerta,^cEugenio Ragazzi^d and Pietro Traldi^a,*
^aCNR, Istituto di Scienze e Tecnologie Molecolari sezione di Padova, Corso Stati Uniti 4, I35127 Padova, Italy. E-mail: pietro.traldi@adr.pd.cnr.it
^bDipartimento di Scienze Farmaceutiche, Università di Padova, Via Marzolo 5, I35100 Padova, Italy
^cIstituto di Clinica Chirurgica Generale II, Università di Padova, Via Giustiniani 2, I35100 Padova, Italy
^dDipartimento di Farmacologia e Anestesiologia, Largo Meneghetti 2, I35131 Padova, Italy

ABSTRACT:

Fourteen blood samples from patients with melanoma and eleven blood samples from healthy subjects were analyzed by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The study was focalized on species of low molecular weight, in the range 800–5000 Da, present in plasma and sera. While for healthy subjects plasma samples lead to the production of a higher number of ionic species, for melanoma patients a high number of diagnostic ions, present with high frequency and with quite high relative abundance, are present in particular in serum samples and to a lesser extent also in plasma. Since plasma samples are obtained more easily in comparison to sera, it is possible to suggest that also plasma can be used for these studies.

Keywords: melanomas, mass spectrometry, MALDI, human plasma, human serum

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