Positive-ion fast atom bombardment tandem mass spectrometry for characterization of sulfated unsaturated disaccharides from heparin and heparan sulfate

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Eur. J. Mass Spectrom. 1, 11 - 22 (1995)

Positive-ion fast atom bombardment tandem mass spectrometry for characterization of sulfated unsaturated disaccharides from heparin and heparan sulfate

Tadashi Ii
Research and Development Laboratories, Soda Aromatic Co., Ltd, Noda shi, Chiba 270-02, Japan
Masayuki Kubota, Takashi Hirano and Mamoru Ohashi^*
Department of Applied Physics and Chemistry, The University of ElectroCommunications, Chofu, Tokyo 182, Japan
Satoshi Okuda
School of Agricultural Science, Nagoya University, Chikusa-ku, Nagoya 464-01, Japan

ABSTRACT:

Positive-ion fast atom bombardment tandem mass spectrometry has been used in the characterization of non-, mono-, di- and trisulfated disaccharides from heparin and heparan sulfate. The positional isomers of sulfate groups ofmono sulfated disaccharides have been distinguished from each other by positive ion fast atom bombardment tandem mass spectra, which provide an easy way of identifying the positional isomers. The fast atom bombardment collision-induced dissociation mass spectrometry/mass spectrometry technique was also applied successfully to the characterization of di and trisulfated disaccharides.

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