Full-text Article (Subscribers only)
Full-text article (280 kB)
(subscribers only)

Buy article on-line and get access immediately
Buy article on-line for £11.75
(get immediate access)

Search
Search

Go Back

Eur. J. Mass Spectrom. 1, 573 - 581 (1995)

Liquid chromatography/electrospray tandem mass spectrometry of amino acid conjugates of jasmonic acid under positive and negative ionisation

Jürgen Schmidt,* Robert Kramell and Gernot Schneider
Institut für Pflanzenbiochemie, Weinberg 3, D-06120 Halle/S., .

ABSTRACT:
The positive and negative ion electrospray mass spectra of a series of (N)-jasmonoyl amino acid conjugates were measured by combined high performance liquid chromatography (HPLC)/mass spectrometry (MS). The mass spectra obtained of both positive and negative ions exhibit mostly a single abundant peak of [M + H]+and [M H], respectively, which indicates the molecular mass of the conjugate. From these ions detailed structural information can be obtained by liquid chromatography/tandem mass spectrometry. The positive daughter ion mass spectra obtained by collision-induced dissociation (CID) of [M+ H]+ give information about both the jasmonoyl moiety and the amino acid component. The negative ion daughter spectra of [M H] are dominated by an ion originating from the deprotonated amino acid. Therefore, the conjugating amino acid can structurally be assigned by both the positive and negative ion CID mass spectra.

Keywords:

You can now buy this paper on-line in PDF format; it costs only £11.75. Just click on the BUY on-line button. You can pay on-line through a secure server and get access immediately.


© IM Publications
Any problems? E-mail .