A strategy for the identification of 2'-deoxynucleoside and 2'-deoxynucleotide adducts using electrospray tandem mass spectrometry

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Eur. J. Mass Spectrom. 2, 181 - 192 (1996)

A strategy for the identification of 2'-deoxynucleoside and 2'-deoxynucleotide adducts using electrospray tandem mass spectrometry

Koen Vanhoutte, Walter Van Dongen and Eddy L. Esmans^*
University of Antwerp (RUCA-UIA), Department of Chemistry, Nucleoside Research and Mass Spectrometry Unit, Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
Elfriede Van den Eeckhout
University of Ghent, Department of Pharmacy, Harelbekestraat 72, B-9000 Gent, Belgium.
Henri Van Onckelen
University of Antwerp (UIA), Department of Biology, Universiteitsplein 1, B-2610 Wilrijk, Belgium.

ABSTRACT:

A methodology using electrospray tandem mass spectrometry (ES MS/MS) was developed to identify 2'-deoxynucleotide adducts of bisphenol A diglycidylether(BPADGE). The 2'-deoxynucleotide adducts were formed in vitro and were separated using reversed phase high pressure liquid chromatography (RP-HPLC). The adducts were investigated by (-) electrospray liquid chromatography-mass spectrometry [()ES LC-MS]. () ES LC-MS/MS allowed the differentiation between 5'-phosphate and base alkylated adducts. More detailed structural information on the heterocyclic moeity was obtained by examining the (+) ES LC-MS/MS data and by comparison with the (+) ES LC-MS/MS spectra of the corresponding 2'-deoxynucleoside-adducts.

Keywords:

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