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Eur. J. Mass Spectrom. 3, 396 - 399 (1997) |
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Letter: Differentiation of isomeric mercapturic acid pathway metabolites of benzo[a]pyrene | ||
Y. Yang, J. Rafter and J.-Å. Gustafsson |
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ABSTRACT: | ||
Isomeric forms of the mercapturic acid pathway metabolites of benzo[a]pyrene (BP) can be differentiated by positive-ion electrospray collision-induced dissociation (CID) mass spectrometry at the nanogram level. The metabolite (+)-r-7,t-8-dihydroxy-t-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE), conjugated at position 10 (bay region) with cysteine, fragments to give characteristic ions at m/z 303 corresponding to a BP-triol cation, these ions are not observed in the CID spectra of BPDEs conjugated at position 7 (non-bay region). | ||
Keywords: benzo[a]pyrene, mercapturic acid, metabolites, diolepoxides, electrospray, tandem mass spectrometry, mass spectrometry, N-acetylcysteine. |
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