New strategy for a rapid connection of multiple disulfide bridges in

w-conotoxins

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Eur. J. Mass Spectrom. 4, 141 - 146 (1998)

New strategy for a rapid connection of multiple disulfide bridges in w-conotoxins

Corinne Loutelier-Bourhis, Hélène Belva and Catherine Lange^*
Spectrométrie de Masse Bio-organique, CNRS-UPRESA 6014, IFRMP 23, Université de Rouen, UFR des Sciences, 76821 Mont-Saint-Aignan Cedex, France.

ABSTRACT:

A strategy for locating multiple disulfide bridges is demonstrated using two MVIIA and MVIIC w-conotoxins in which the locations of disulfide bridges are known. The method is based on the use of selective trypsin digestion followed by non-selective pronase digestion in combination with electrospray mass spectrometry. This strategy promises to be a suitable first step in investigations applied to new peptides of unknown structure.

Keywords: Electrospray, disulfide bridges, conotoxins, pronase

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