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Eur. J. Mass Spectrom. 4, 223 - 232 (1998)

Origins of the proton in the generation of protonated polymers and peptides in matrix-assisted laser desorption/ionization

Carol K.L. Wong, M.P. So and T-W. Dominic Chan
Department of Chemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong.

ABSTRACT:
In this paper, various possible sources of protons for protonation of polymer and peptide molecules under matrix-assisted laser desorption/ionization conditions were studied by using a deuterium labeling method. It was demonstrated that exchangeable protons from either carboxylic acid or hydroxyl groups of the matrix were important, but not essential, for protonation of the analyte. For a nitroanthracene matrix, the non-exchangeable protons from the matrix and the exchangeable protons from the solvent (and possibly from the analyte) were also shown to contribute protons for the formation of protonated molecule-ions.

Keywords: MALDI, protonation, ionization, proton source, deuterium labeling, proteins, polymers, ion formation.

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