High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry of phospholipids in

Natronobacterium magadii

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Eur. J. Mass Spectrom. 5, 151 - 156 (1999)

High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry of phospholipids in Natronobacterium magadii

Di-Fei Qiu^* and Xiao-Yao Xiao
Department of Chemistry, Hunan University, Hunan Changsha 410082, People's Republic of China.
Terence J. Walton
Biochemistry Research Group, School of Biological Sciences, University of Wales Swansea, Swansea SA2 8PP, UK.
Marguerite P.L. Games and David E. Games
Mass Spectrometry Research Unit, University of Wales Swansea, Swansea SA2 8PP, UK..

ABSTRACT:

A novel method of coupled liquid chromatography/atmospheric pressure ionization mass spectrometry (LC/APCI-MS) has been used to analyze archaebacterium phospholipids in Natronobacterium magadii. Four major lipids, C₂₀-C₂₀ and C_{20
-C₂₅ PGP-Me and PG were found to exist in this organism. APCI mass spectra provided ample information for elucidation of the lipid structures. As an example, the positive-ion fragmentation of C₂₀-C₂₀ PGP-Me is discussed. In our study, LC/APCI-MS was shown to be an effective method in both the positive- and negative-ion modes for the direct mass spectrometric analysis of archaebacterium phospholipids.}

Keywords: atmospheric pressure chemical ionization, phospholipids, archaebacteria, Natronobacterium magadii, mass spectrometry.

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