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Eur. J. Mass Spectrom. 5, 165 - 168 (1999) |
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Letter: The use of electrospray ionization mass spectrometry to distinguish the lot-to-lot heterogeneity of an antigen specific monoclonal antibody from a specific cellular clone | ||
Maciej Adamczyk*, John C. Gebler, Charles A. Harrington and Austin F. Sequeira |
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ABSTRACT: | ||
Liquid chromatography/electrospray ionization mass spectrometry was used to investigate eight different preparations of an anti-thyroid stimulating hormone murine monoclonal antibody, obtained from a single cellular clone grown under identical conditions. While the spectra of the intact IgG provided a primary fingerprint distinguishing the investigated lots, subsequent analysis of the antibody fragments further confirmed the differences. | ||
Keywords: electrospray ionization, monoclonal antibodies, fingerprinting. |
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