Identification of bound alcohols in soil humic acids by gas chromatography-mass spectrometry

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Eur. J. Mass Spectrom. 6, 439 - 441 (2000)

Identification of bound alcohols in soil humic acids by gas chromatography-mass spectrometry

Gersende Berthier
Collège Louisa Paulin, F-81120 Realmont, France
Sen Dou
Department of Soil Science, Jilin Agricultural University, Changchun, Peoples Republic of China
Torren Peakman
School of Chemistry, University of Bristol, Cantocks Close, Bristol BS8 lTS, UK
Eric Lichtfouse^*
Soil-Water Geochemistry and Microbiology Laboratories, Earth Sciences - CST, University of Burgundy - INRA, 6, Boulevard Gabriel, F-21000 Dijon, France. E-mail:

ABSTRACT:

Humic acids are complex, partly macromolecular, yellow-brownish substances occurring in soils, waters and sediments. In order to shed some light on their molecular structure, crop humic acids were cleaved by alkaline hydrolysis (KOH). The products were fractionated by thin layer chromatography to give mono-alcohols which were analysed as acetate derivatives by gas chromatography coupled to mass spectrometry. Linear alcohols, sterols, stanols and plant-derived triterpenoid alcohols were identified by co-injection of pure standards and by comparison with literature data. These findings imply that alcohols could have been incorporated into the humic matrix by esterification with carboxylic acids. Furthermore, the presence of stanols as hydrogenated counterparts of sterols suggests that a process of hydrogenation is operating in soils.

Keywords: chemical degradation, humic substances, GC-MS, sterol, fatty alcohol, triterpenoid

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