Structural elucidation of eighteen cerebrosides from

Holothuria coronopertusa in a complex mixture by high-energy collision-induced dissociation of [M + Li]⁺ ions

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Eur. J. Mass Spectrom. 7, 409 - 417 (2001)

Structural elucidation of eighteen cerebrosides from Holothuria coronopertusa in a complex mixture by high-energy collision-induced dissociation of [M + Li]⁺ ions

Nathalie Hue, Alain Montagnac, Mary Païs,^* Laurent Serani and Olivier Laprévote^*
Institut de Chimie des Substances Naturelles, CNRS, Avenue de la Terrasse, 91 198 Gif-sur-Yvette Cedex, France. E-mail:
Jean-Louis Menou and Cécile Debitusa
Centre IRD, BP A5, Nouméa, New Caledonia

ABSTRACT:

Eighteen cerebrosides were detected in admixture from the sea cucumber Holothuria coronopertusa. Their structures have been established on the basis of liquid-secondary ion mass spectrometry (LSIMS) experiments. The collision-induced dissociation (CID) of the lithiated molecules ([M + Li]⁺) led to diagnostic fragment ions which were further identified by tandem mass spectrometry (MS/MS). In the present study, the combination of LSIMS as ionization mode and high-energy MS/MS analysis appears to be a very powerful method for the rapid analysis of unresolved mixtures of complex molecules.

Keywords: liquid secondary ion mass spectrometry, tandem mass spectrometry, cerebrosides, Holothuria coronopertusa

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