Analysis of oligomeric and polymeric tannins of grape berries by liquid chromatography/electrospray ionization multiple-stage tandem mass spectrometry

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Eur. J. Mass Spectrom. 7, 419 - 426 (2001)

Analysis of oligomeric and polymeric tannins of grape berries by liquid chromatography/electrospray ionization multiple-stage tandem mass spectrometry

Camille Perret and Raphael Tabacchi
Institut de Chimie, Université de Neuchâtel, Av. De Bellevaux 51, 2000 Neuchâtel, Switzerland
Roger Pezet^*
Station Fédérale de Recherches en Production Végétale de Changins, Route de Duillier, 1260 Nyon, Switzerland

ABSTRACT:

Tannins are highly polar, water-soluble and thermally-labile compounds which are difficult to ionize. Mass spectrometry is very useful for the structural determination of such compounds. In this article, we describe a sensitive method for the identification of tannic composition in grape berries (var. Gamaret). An ion trap, equipped with an electrospray ionization (ESI) interface, allowed us to develop multiple-stage fragmentation (MSⁿ), which provided useful structural information. This technique was applied to a mixture of roanthocyanidins and proanthocyanidin gallate oligomers (B-type) generating identical ion sequences for each parent ion.

Keywords: tannins, proanthocyanidins, proanthocyanidin gallates, grapes, HPLC, ESI-MS, MSⁿ, LC/MS

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