Gas chromatograhy-tandem mass spectrometry determination of 8-iso-PGF2α, a biomarker of

in vivo lipid peroxidation, in human plasma and urine

Full-text article (196 kB)
(subscribers only)

Buy article on-line for £11.75
(get immediate access)

Eur. J. Mass Spectrom. 7, 427 - 432 (2001)

Gas chromatograhy-tandem mass spectrometry determination of 8-iso-PGF2α, a biomarker of in vivo lipid peroxidation, in human plasma and urine

M. Richelle, M.E. Turini, R. Guidoux, I. Tavazzi, S. Métairon and L.B. Fay^*
Nestlé Research Center, Nestec Ltd, Vers-chez-les-Blanc, PO Box 44, 1000 Lausanne 26, Switzerland. E-mail:

ABSTRACT:

The measurement of isoprostanes is a promising assay that is specific and sensitive enough to detect in vivo lipid peroxidation. We present here a gas chromatography-tandem mass spectrometry (GC/MS/MS) method that enables determination of 8-iso-prostaglandin F2α (8-iso-PGF2α) in human plasma and urine. After the addition of [²H₄]-PGF2α as the internal standard to acidified plasma or urine, the samples are purified on C₁₈ and silica cartridges, derivatised as pentafluorobenzyl esters, extracted with diethyl ether, purified on silica gel TLC plates and finally silylated. Then, 8-iso-PGF2α and its internal standard are measured by GC/MS/MS in selective-reaction monitoring mode using the transition [M 181] to [M 181 (3 x 90)]. The detection limit of this method is 5 pg mL¹. Its application is presented in two situations of oxidative stress: in vitro low-density lipoprotein oxidation and in smokers. Measurement of urinary 8-iso-PGF2α levels provides a non-invasive in vivo index of free radical generation that appears not to be confounded by changes in diet.

Keywords: oxidative stress, lipid peroxidation, isoprostane, GC/MS/MS

You can now buy this paper on-line in PDF format; it costs only £11.75. Just click on the BUY on-line button. You can pay on-line through a secure server and get access immediately.