Detection and characterization of natural polyamines by high-performance liquid chromatography-atmospheric pressure chemical ionization (electrospray ionization) mass spectrometry

Full-text article (827 kB)
(subscribers only)

Eur. J. Mass Spectrom. 8, 1 - 16 (2002)

Detection and characterization of natural polyamines by high-performance liquid chromatography-atmospheric pressure chemical ionization (electrospray ionization) mass spectrometry

Sergiy Chesnov, Laurent Bigler and Manfred Hesse^*
Institute of Organic Chemistry, University of Zürich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. E-mail:

ABSTRACT:

Account: The application of high-performance liquid chromatography on-line coupled with atmospheric-pressure chemical-ionization mass spectrometry (HPLC-APCI-MS), electrospray-ionization mass spectrometry (ESI-MS), as well as with tandem mass spectrometry (MS/MS), for the analysis of natural products is discussed. The separation, detection and structure elucidation of polyamine-containing compounds contained in plant material and spider venoms was performed by means of these techniques. The use of the methods for biochemical investigations of diverse natural materials is proposed.

Keywords: tandem mass spectrometry, spider, venom, aphelandrine, spermine, spermidine, biosynthesis, phenol oxidation, labeled alkaloids

You can now buy this paper on-line in PDF format; it costs only £11.75. Just click on the BUY on-line button. You can pay on-line through a secure server and get access immediately.