|
Eur. J. Mass Spectrom. 8, 117 - 121 (2002) |
|
Electronic excitation gives informative fragmentation of polypeptide cations and anions | ||
K.F. Haselmann, B.A. Budnik, F. Kjeldsen, M.L. Nielsen, J.V. Olsen and R.A. Zubarev* |
||
ABSTRACT: | ||
A Fourier transform mass spectrometer is a versatile instrument with a range of available fragmentation techniques. Comparison of polypeptide fragmentation patterns revealed that the techniques involving electronic excitation, such as hot-electron-capture dissociation (HECD) and electron-detachment dissociation (EDD), are even more informative than vibrational excitation (VE) techniques such as collisional activation. For dications of the peptide KIMHASELMANN, 11 eV HECD cleaved all inter-residue links in at least two places, with up to five fragments characterizing each link. For dianions of the same molecule, VE produced only one backbone cleavage whereas EDD gave ten, including five internal cleavage fragments. This is consistent with the general postulate that homogeneous electronic excitation yields more types of cleavage than near-equilibrium processes such as VE. | ||
Keywords: polypetides, fragmentation, electronic excitation, ion&150;electron reactions, electron capture dissociation, collision-induced dissociation, electron-detachment dissociation |
You can now buy this paper on-line in PDF format; it costs only £11.75. Just click on the BUY on-line button. You can pay on-line through a secure server and get access immediately.
© IM Publications
Any problems? E-mail .