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Eur. J. Mass Spectrom. DOI: 10.1255/ejms.514

Capillary electrophoresis and electron capture dissociation Fourier transform ion cyclotron resonance mass spectrometry for peptide mixture and protein digest analysis

Youri O. Tsybin,a Magnus Wetterhall,b Karin E. Markides,b Per Håkanssona and Jonas Bergquistb*
aDivision of Ion Physics, The Ångström Laboratory, Uppsala University, Box 534, SE 751 21, Uppsala, Sweden
bDepartment of Analytical Chemistry, Uppsala University, Box 531, SE 751 21, Uppsala, Sweden

ABSTRACT:
Recent advances in peptide fragmentation techniques and mass spectrometry have opened up the possibility of combining peptide separation techniques, such as capillary electrophoresis and capillary liquid chromatography, with Fourier transform ion cyclotron resonance and electron capture dissociation in order to characterise peptide mixtures and protein digests. The results presented in this study show that CE/ECD-FTICR MS can be employed for peptide characterisation in mixtures of standard peptides and peptides resulting from the enzymatic digestion of proteins. Furthermore, the technique has potential for the identification and localisation of post-translational modifications in peptides and proteins.

Keywords: protein, peptide mixture, enzymatic digest, trypsin, capillary electrophoresis, mass spectrometry, Fourier transform ion cyclotron resonance, electron capture dissociation, sequencing, post-translational modifications


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