Full-text Article (Subscribers only)
Full-text article (555 kB)
(subscribers only)

Search
Search

Go Back

Eur. J. Mass Spectrom. DOI: 10.1255/ejms.526

Structural characterization by both positive and negative electrospray mass spectrometry of partially methyl-esterified oligogalacturonides puriefied by semi-preparative high performance anion exchange chromatography

Bernard Quéméner, Cédric Désiré and Marc Lahaye
Institut National de la Recherche Agronomique, Unité de Recherche sur les Polysaccharides, leurs organisations et leurs Interatctions (URPOI), rue de la Géraudiêre, BP 71627, 44316 Nantes, France
Laurent Debrawer
Institut National de la Recherche Agronomique, Laboratoire des xênobiotiques, chemin de Tournefeuille, St-Martin-du-Touch, BP 3, 31931 Toulouse, France
Luc Negroni
Institut National de la Recherche Agronomique, Plate-forme de protéomique, IFR87, Ferme du Moulon, 91190 Gif-sur-Yvette, France

ABSTRACT:
The off-line coupling of high performance anion exchange chromatography (HPAEC) to electrospray ionization/ion trap mass spectrometry (ESI-ITMS) is described. The Dionex Carbohydrate Membrane Desalter (CDM) has been assessed as an on-line chromatographic desalting system to remove the high sodium concentration necessary for the HPAEC separation of partially methyl-esterified oligogalacturonides. The developed HPAEC configuration proved to be suitable for the indirect coupling with ESI-ITMS. This paper provides some interesting features of positive and negative MSn analysis of these acidic oligosaccharides. The spectra acquired in both negative- and positive-ion modes show characteristic fragment ions resulting from glycosidic bond and cross-ring cleavages. Some new MS fragmentation routes are also described. The positive-ion mode gave more complex spectra but was as informative as the negative one. ESI-ITMS revealed to be, as previously reported directly on non-separated enzymatic digest, a powerful sequencing technique for the determination of the linkage type and the methylester distribution of partially methyl-esterified oligogalacturonides. Moreover, contrary to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, it gives valuable information on the elution behavior of these oligomers in relation to their structure, namely the HPAEC coelution of isomeric structures

Keywords: pectins, endogalacturonase, partially-esterified oligogalacturonides, structural characterization, HPAEC, ESI-ITMS


© IM Publications
Any problems? E-mail .
CrossRef Member