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Eur. J. Mass Spectrom. DOI: 10.1255/ejms.572 |
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Characterisation of the intact rainbow trout vitellogenin protein and analysis of its derived tryptic and cyanogen bromide peptides by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and electrospray ionisation Q time-of-flight mass spectrometry | ||
Joseph Banoub,a,b* Pierre Thibault,c Alejandro Cohen,b Atef Mansour,a David H. Heeleyb and Donna Jackmanb |
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ABSTRACT: | ||
Vitellogenin (VTG) is a protein produced by the liver of oviparous animals. It is being used as a biomarker for exposure to endocrine disruptors in many species. Rainbow Trout Vtg has recently been sequenced by conventional cDNA nucleotide approach. We focused on protein characterisation of the intact protein and its derived tryptic and cyanogen bromide peptides by matrix-assisted laser desorption/ionisation and electrospray ionization mass spectrometry. The molecular mass of the intact protein was found to be 183127 Da. A large number of unidentified peptide ions encourage further structural analysis to propose possible sequence variants and post translational modifications. | ||
Keywords: vitellogenin, characterization, cyanogen bromide, tryptic, MALDI MS, ESI MS, fingerprint |
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