Letter: Collision-induced dissociation of peptide thioesters: influence of the peptide length on the fragmentation

Eur. J. Mass Spectrom. 11, 31–34 (2005)
DOI: 10.1255/ejms.709

Letter: Collision-induced dissociation of peptide thioesters: influence of the peptide length on the fragmentation

Corinne Buré,^a,* Olivier Boujard,^a Marylène Bertrand,^a Catherine Lange^b and Agnès F. Delmas^a,*
^aCentre de Biophysique Moléculaire, CNRS UPR 4301#, rue Charles Sadron, 45071 Orléans Cedex 02, France
^bSpectrométrie de Masse Bio-organique, CNRS-UMR 6014, Université de Rouen, (F) 76821 Mont Saint Aignan cedex

ABSTRACT:

Five peptide thioesters of increasing length were fragmented under two processes, in-source and in-collision cell fragmentation, using an electrospray source coupled to a triple quadrupole. Comparison of their fragmentations was made in regard to the length. The two fragmentation conditions show that the peptide length has no influence on structural information and that the fragmentation efficiency is higher for the smallest peptides than for the longest. The particularity of these peptide thioesters consists on the neutral loss of ethanethiol. The absence of the a3 fragment ion and the presence of the (a₃-17) ion on the CID mass spectra are noted.

Keywords: peptide thioesters, fragmentation, peptide length

You can buy this paper on-line in PDF format; it costs only £11.75. Just click on the BUY on-line button. You can pay on-line through a secure server and get access immediately.