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Renfang Song,a,b Wenbing Zhang,a Huayong Chen,a,b Huimin
Ma,a,b Yulian Dong,a Guoying Sheng,a Zhen Zhoua and Jiamo Fua,*
aState Key Laboratory
of Organic Geochemistry, Guangzhou Research Center of Mass Spectrometry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
E-mail: fujm@gig.ac.cn
bGraduate School of the Chinese Academy of Sciences, Beijing, China |
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Three groups of four oligonucleotides with special single
nucleotide polymorphisms (SNP) sites in exon 4 of the p53 gene were analyzed with ion-pair reversed-phase high-performance liquid chromatography electrospray ionization mass
spectrometry tandem mass spectrometry. The retention order of four oligonucleotides with SNPs was C < G < A < T, regardless of whether the polymorphisms were at the
3′ end, the 5′ end, or the middle of the oligonucleotides. The charge state of the molecular ion affects the MS/MS spectra of the oligonucleotides. SNPs at the
3′ end can be easily identified from the fragmentation pattern of the 2- charge state, but not from the 3- charge state, especially from the w1 fragment. The single base
may be taken as the symbol of the 5′ end SNP site derived from [M–3H]2–, but not from the [M–3H]2– charge state.
The oligonucleotides with SNPs in the middle were also determined from the [M–2H]2– precursor ion. |
