The following papers have been published in the Special Issue [Vol. 7 No. 2 (2001)] on the application of mass spectrometry to proteomics.

Individual papers can be bought on-line for £11.75 or the whole issue can be ordered.

Editorial: Mass spectrometry of proteomics

Peter J. Derrick and
University of Warwick
Scott D. Patterson
Celera Genomics

Full-text article

Toward efficient analysis of >70 kDa proteins with 100% sequence coverage

Andrew J. Forbes, Matthew T. Mazur and Neil L. Kelleher^*
Department of Chemistry, 600 S. Mathews Avenue, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA E-mail: kelleher@scs.uiuc.edu
Hiten M. Patel and Christopher T. Walsh
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA

Full-text article (243 kB)      Abstract     Buy article on-line

Gas-phase conformation can have an influence on peptide fragmentation

William J. Griffiths^* and Andreas P. Jonsson
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-17177 Stockholm, Sweden. E-mail: william.griffiths@mbb.ki.se

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Analysis of protein sequences and protein complexes by matrix-assisted laser desorption/ionization mass spectrometry

Maya Belghazi and Katell Bathany
Institut Européen de Chimie Biologie, 33607 Pessac, France
Codjo Hountondji
Laboratoire de Biochimie, UMR CNRS 7654, Ecole Polytechnique, 91128 Palaiseau, France
Xavier Grandier-Vazeille and Stéphen Manon
Institut de Biologie et Génétique Cellulaire du CNRS, 33077 Bordeaux, France
Jean-Marie Schmitter^*
Laboratoire de Physico-Toxico-Chimie, UMR CNRS 5472 Université Bordeaux I, 33405 Talence, France

Full-text article (176 kB)      Abstract     Buy article on-line

Simplified sample preparation method for protein identification by matrix-assisted laser desorption/ionization mass spectrometry: in-gel digestion on the probe surface

Allan Stensballe and Ole Nørregaard Jensen^*
Department of Biochemistry & Molecular Biology, University of Southern Denmark, Odense University, Campusvej 55, DK-5230 Odense M, Denmark. E-mail: jenseno@bmb.sdu.dk

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Improved accuracy in the matrix-assisted laser desorption/ionisation mass spectrometry determination of the molecular mass of cyanogen bromide fragments of proteins by post-cleavage reaction with tris(hydroxymethyl)aminomethane

A. Compagnini, V. Cunsolo, S. Foti and R. Saletti^*
Dipartimento di Scienze Chimiche, Università degli Studi di Catania, Viale A. Doria, 6, 95125 Catania, Italy. E-mail: sfoti@dipchi.unict.it

Full-text article (131 kB)      Abstract     Buy article on-line

Limited acid hydrolysis as a means of fragmenting proteins isolated upon ProteinChip^® Array surfaces

Shanhua Lin, Pete Tornatore and Scot R. Weinberger^*
Ciphergen Biosystems, Inc., 6611 Dumbarton Circle, Fremont, CA 94555, USA. E-mail: sweinberger@ciphergen.com
Daniel King and Ron Orlando
CCRC, University of Georgia, Athens, GA 30602, USA

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Integrated system for high-throughput protein identification using a microfabricated device coupled to capillary electrophoresis/nanoelectrospray mass spectrometry

Jianjun Li, Tammy-Lynn Tremblay and Pierre Thibault^*
Institute for Biological Sciences, 100 Sussex Dr., Ottawa, Ontario, Canada K1A 0R6 E-mail: pierre.thibault@nrc.ca
Can Wang, Said Attiya and D. Jed Harrison
Department of Chemistry, University of Alberta, Edmonton, AB, Canada T6G 2G2

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Investigation of the applicability of a sequential digestion protocol using trypsin and leucine aminopeptidase M for protein identification by matrix-assisted laser desorption/ionization mass spectrometry

Alan Doucette and Liang Li^*
Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada, T6G 2G2. E-mail: Liang.Li@ualberta.ca

Full-text article (235 kB)      Abstract     Buy article on-line

Multiple separations facilitate identification of protein variants by mass spectrometry

Zhongli Zhang, David L. Smith and Jean B. Smith^*
Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304, USA E-mail: jsmith8@unl.edu

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Mass spectrometric characterization of proteins extracted from Jurkat T cell detergent-resistant membrane domains

Priska D. von Haller, Sam Donohoe, Ruedi Aebersold and Julian D. Watts^*
Department of Molecular Biotechnology, University of Washington, Seattle, WA 98195, USA E-mail: julian@systemsbiology.org
David R. Goodlett
Institute for Systems Biology, 4225 Roosevelt Way, Suite 200, Seattle, WA 98105-6099, USA

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The effects of transcription regulating genes PDR1, pdr1-3 and PDR3 in pleiotropic drug resistance

Arkadiusz Nawrocki, Stephen J. Fey and Peter Mose Larsen^*
Centre for Proteome Analysis in Life Sciences, International Science Park Odense, University of Southern Denmark, Forskerparken 10B, 5230 Odense M, Denmark. E-mail: ps@cpa.spo.dk
André Goffeau
Chaire Internationale Blaise Pascal, Laboratoire Genetique Moleculaire, Ecole Normale Superieure, 46 rue d’Ulm, 75230 Paris Cedex 05, France
Peter Roepstorff
Department of Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark

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Structural modifications of Methanococcus jannaschii flagellin proteins revealed by proteome analysis

Carol S. Giometti,^* Sandra L. Tollaksen and Gyorgy Babnigg
Biosciences Division, Argonne National Laboratory, 9700 South Cass Avenue, Building 202, Room B117, Argonne, IL 60439, USA. E-mail: csgiometti@anl.gov
Claudia I. Reich and Gary J. Olsen
Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA
Hanjo Lim and John R. Yates, III
Mass Spectrometry Group, The Scripps Institute, La Jolla, CA, USA

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Detection and localisation of disulphide bonds in a synthetic peptide reproducing the sequence 1–30 of Par j 1.0101 by electrospray ionisation mass spectrometry

Vincenzo Cunsolo, Salvatore Foti and Rosaria Saletti
Dipartimento di Scienze Chimiche, Università di Catania, Viale A. Doria 6, I-95125 Catania, Italy
Leopoldo Ceraulo^* and Vita di Stefano
Dipartimento di Chimica e Tecnologie Farmaceutiche, Università di Palermo, Via Archirafi 32, I-90123 Palermo, Italy E-mail: lceraulo@unipa.it

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