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Edited by Guido Grandi
While the sequence of the human genome sequence has hit the headlines, extensive exploitation of this for practical applications is still to come. Genomic and post-genomic technologies applied to viral and bacterial pathogens, which are almost equally important from a scientific perspective, have the potential to be translated into useful products and processes much more rapidly.
Genomics, Proteomics and Vaccines introduces the history of vaccinology and discusses how vaccines are expected to evolve in the future. It describes the relevant technologies, including genome sequencing and analysis, DNA microarrays, 2D electrophoresis and 2D chromatography, mass spectrometry and high-throughput protein expression and purification. The book also features examples of the exploitation of genomics and post-genomics in vaccine discovery, and contains useful descriptions of the biology and pathogenesis of clinically important bacterial pathogens.
Hardback
Published: 2003
Pages: 320
Contents
Section I Introduction
1 Vaccination: past, present and future
Maria Lattanzi and Rino Rappuoli
2 Bioinformatics, DNA microarrays and proteomics in vaccine discovery: competing or complementary technologies?
Guido Grandi
Section II Technologies
3 Genome sequencing and analysis
Hervé Tettelin and Tamara Feldblyum
4 Understanding DNA Microarrays: Sources and Magnitudes of Variances in DNA Microarray Data Sets
She-pin Hung, G. Wesley Hatfield, Suman Sundaresh and Pierre F. Baldi
5 The proteome, anno domini two zero zero three
Pier Giorgio Righetti, Mahmoud Hamdan, Frederic Reymond and Joël and S. Rossier
6 Mass spectrometry in proteomics
Pierre-Alain Binz
7 High Throughput Cloning, Expression and Purification Technologies
Andreas Kreusch and Scott A. Lesley
Section III Applications
8 Meningococcus B: from Genome to Vaccine
Davide Serruto, Rino Rappuoli and Mariagrazia Pizza
9 Vaccines Against Pathogenic Streptococci
John L. Telford, Immaculada Margarit y Ros, Domenico Maione, Vega Masignani, Hervé Tettelin, Giuliano Bensi and Guido Grandi
10 Proteome analysis of outer membrane and extracellular proteins from Pseudomonas aeruginosa for vaccine discovery
Stuart J. Cordwell and Amanda S. Nouwens
11 Proteomics and anti-Chlamydia vaccine discovery
Gunna Christiansen, Svend Birkelund, Brian B. Vandahl and Allan C Shaw
12 Searching the Chlamydia genomes for new vaccine candidates
Giulio Ratti, Oretta Finco and Guido Grandi
13 Identification of the “antigenome” – a novel tool for design and development of subunit vaccines against bacterial pathogens
Eszter Nagy, Tamás Henics, Alexander von Gabain and Andreas Meinke
Index
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