by Chhabil Dass
A practical introduction to contemporary instrumentation, techniques, and applications.
This book provides professionals in the biological sciences with a solid basis in the fundamentals of contemporary mass spectrometry so that they can confidently choose and apply the best instrumentation and experimental approaches for their needs. The only single-volume guide of its kind, Principles and Practice of Biological Mass Spectrometry offers comprehensive coverage of the latest instrumentation, techniques, and applications in the rapidly expanding field of biological mass spectrometry, making it an excellent working resource for experts and novices alike.
Principles and Practice of Biological Mass Spectrometry begins with a detailed review of the fundamentals of and recent developments in biological mass spectrometry instrumentation. This is followed by practical, well-illustrated instances of important mass spectrometric techniques, including:
* Tandem mass spectrometry
* Collision-induced dissociation
* Molecular mass determination
* Coupling of mass spectrometry with separation techniques
* Quantification
The final portion of the book is devoted to an exploration of contemporary approaches to the characterization of the various classes of compounds relevant to the biological sciences. While the emphasis is on proteins and peptides, nucleic acid, carbohydrates, and lipids, pharmaceutical drugs and metabolites also receive extensive coverage. Throughout, coverage is generously supplemented with examples of applications across a wide range of commercial and academic venues.
Principles and Practice of Biological Mass Spectrometry is an invaluable working resource for chemists, biochemists, biologists, molecular biologists, biomedical researchers, pharmacologists, immunologists, forensic scientists, environmental scientists, and all whose research involves the analysis of biological compounds.
Hardback
Published: 2000
Pages: 448
Contents
Introduction to Mass Spectrometry
Ionization Methods
Mass Analysis and Ion Detection
Tandem Mass Spectrometry
Molecular Mass Measurement
Quantitative Analysis
Coupling of Separation Techniques with Mass Spectrometry
Structural Analysis of Proteins
Structural Characterization of Peptides
Analysis of Glycoproteins and Oligosaccharides
Folding—Unfolding and Higher-order Structures of Proteins
Investigation of Noncovalent Interactions
Structure Determination of Lipids and Glycolipids
Screening Combinatorial Libraries
Characterization of Oligonucleotides
Applications to Real-World Problems
Index
|